Journal: Cells

Article Title: Low-Dose rIL-15 Protects from Nephrotoxic Serum Nephritis via CD8 + T Cells

doi: 10.3390/cells11223656

Figure Lengend Snippet: IL-15 increases CD44 + memory CD8 + T cell numbers in the periphery and mitigates a cytotoxic phenotype. Data represent day 7 of NTS. Quantification of ( A ) CD45 + CD19 - CD3 - NK1.1 + NK cells, ( B ) CD45 + CD19 - CD3 + NK1.1 + NKT cells, and ( C ) CD45 + CD19 - CD3 - tet-CD1d + iNKT cells in kidney tissue. Quantification of ( D ) CD45 + CD3 + CD8 + T cells, ( E ) CD44 + CD8 + memory T cells, ( F ) CD122 + memory CD8 + T cells, and ( G ) Ly49 + CD122 + memory CD8 + T cells in the lymph node. ( H ) Relative gene expression of Prf1 , Gzmb , Ifnγ and ( I ) Ikzf2 , Tgfb1 , and Foxp3 in sorted CD8 + T cells after 4 days of culture. Flow cytometry analysis of frequencies of ( J ) CD8 + and ( K ) IFNγ + cells of CD8 + T cells and ( L ) representative plots of splenocytes after 4 days of culture. Data in H-L represent two independent experiments. Black dots represent individual values of respective mice. * p ≤ 0.05, ** p ≤ 0.01. Statistical analysis used was Student’s t -test or Mann–Whitney test. All data are mean ± SEM.

Article Snippet: To assess the relative gene expression in CD8 + T cells, negative MACS sorted cells were cultured (1 × 10 6 cells per ml, in 2.5 mL, 1 well per sample) in RPMI with 10% FCS, 1%Pen/Strep, and stimulated with Dynabeads ® Mouse T-Activator CD3/CD28 (Gibco by Life Technologies, Carlsbad, CA, USA) according to the standard protocol for 4 days.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY